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pet 29b vector  (Addgene inc)


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    Addgene inc pet 29b vector
    Pet 29b Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgemhe+vector/pmc12711892-519-10-12?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    pet 29b vector - by Bioz Stars, 2026-06
    93/100 stars

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    NHA1is electroneutral and does not exhibit voltage-dependence. Nha1 was expressed in Xenopus laevis oocytes and compared to uninjected oocytes (Empty control). Currents activated by the depicted voltage-clamp protocol in Kulori’s solution (control), in 0 mM K + , 0 mM Na + and in pH 8.5. ( A ) Voltage-clamp protocol and representative currents recorded from empty controls and Nha1 injected oocytes. ( B ) Mean currents plotted as a function of voltage, n=6-8.

    Journal: bioRxiv

    Article Title: NHA1 is a cation/proton antiporter essential for the water-conserving functions of the rectal complex in Tribolium castaneum

    doi: 10.1101/2022.09.29.510179

    Figure Lengend Snippet: NHA1is electroneutral and does not exhibit voltage-dependence. Nha1 was expressed in Xenopus laevis oocytes and compared to uninjected oocytes (Empty control). Currents activated by the depicted voltage-clamp protocol in Kulori’s solution (control), in 0 mM K + , 0 mM Na + and in pH 8.5. ( A ) Voltage-clamp protocol and representative currents recorded from empty controls and Nha1 injected oocytes. ( B ) Mean currents plotted as a function of voltage, n=6-8.

    Article Snippet: The PCR products were subsequently cloned into pGEMHE Xenopus laevis oocyte expression vector using In-Fusion ® HD cloning kit (TaKaRa Bio Inc, Kusatsu, JP) and the final sequence validated (Eurofin, Luxemburg, LU).

    Techniques: Injection